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M9490333.TXT
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1994-09-19
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Document 0333
DOCN M9490333
TI [The isolation and characteristics of the green monkey lentivirus]
DT 9411
AU Rezikian SA; Voevodin AF; Klots IN; Shtutman MS; Lapin BA
SO Vopr Virusol. 1993 Mar-Apr;38(2):76-8. Unique Identifier : AIDSLINE
MED/94337516
AB We characterized the simian immunodeficiency virus isolated from
Cercopithecus aethiops (subspecies C. a. pygerythrus) originating from
Kenya. SIV was isolated and continuously produced with the MOLT4 clone 8
cell line and was designated as SIV-SU1. SIV-SU1 isolate replicated with
high efficiency in MOLT4 clone 8, MT-2 with moderate efficiency in CEM x
174 and with poor efficiency in HUT-78, U937, C8166. The infection of
MT-2, C8166 and HUT-78 resulted in extensive cell killing. Western
blotting of purified preparations of SIV-SU1 revealed viral proteins of
130, 68, 55, 41, 24, 17 kDa. Cross-reactivity of SIV-SU1 proteins with
HIV-1, HIV-2, SIVmac, SIVsm, SIVmnd was studied by
radioimmunoprecipitation assay. The most extensive cross-reactivity was
observed with SIVmac. Total cellular DNA from chronically infected cells
was hybridized to SIVagm266 DNA probes. Detection of cross-hybridizing
DNA sequences required very low stringency, and the restriction
endonuclease fragmentation pattern of SIV-SU1 differed from other SIVs.
DE Animal Antigens, Viral/ANALYSIS Cercopithecus aethiops/*MICROBIOLOGY
Cross Reactions DNA, Viral/GENETICS Electrophoresis, Polyacrylamide
Gel English Abstract Human Immunoblotting Lymphocytes/MICROBIOLOGY
Radioimmunoassay Restriction Mapping
SIV/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF/PHYSIOLOGY Virus Cultivation
Virus Replication JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).